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Copy file name to clipboardExpand all lines: internal_use/docs/source/BeginnerSegmentation/BeginnerSegmentationPlugins.md
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@@ -11,7 +11,7 @@ Imaging Platform, Broad Institute of MIT and Harvard, Cambridge, MA.
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This will happen automatically the first time you call a given Docker from CellProfiler (i.e. run a CellProfiler pipeline that uses the Docker) but if you are running this tutorial in a workshop setting we strongly recommend download in advance of the workshop as these are large files (5-10 GB) and bandwidth is often limited in a workshop setting.
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In Docker Desktop or Podman Desktop you can search for containers in the top search bar (see below).
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Make sure you select a tag (version) that is supported by the plugin you are using and then select "Pull".
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We reccomend`biocontainers/ilastik:1.4.0_cv2` for ilastik and `cellprofiler/runcellpose_with_pretrained:3.1.2.2` or `cellprofiler/runcellpose_with_pretrained:4.0.6` for Cellpose.
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We recommend`biocontainers/ilastik:1.4.0_cv2` for ilastik and `cellprofiler/runcellpose_with_pretrained:3.1.2.2` for Cellpose.
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1. Download the example images from the Beginner Segmentation Tutorial.
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[Click here for download](https://github.com/CellProfiler/tutorials/raw/master/BeginnerSegmentation/Archive_EN.zip).
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(Delete/ignore the pipelines; we will just use the images.)
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### Load the pipeline and evaluate segmentation
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```{note}
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If using Docker, the very first time you execute the RunCellpose module, it will need to download a ~5GB-10GB file (depending on the verison of Cellpose), which may be slow depending on your internet connection (unless you downloaded it in preparation as we suggest above).
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If using Docker/Podman, the very first time you execute the RunCellpose module, it will need to download a ~5GB-10GB file (depending on the verison of Cellpose), which may be slow depending on your internet connection (unless you downloaded it in preparation as we suggest above).
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You only need to do this step once, however!
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```
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1. Drag `bonus_3_cellpose.cppipe` into the pipeline panel.
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1. Drag the `images_Illum-corrected` subfolder from the main exercise into the Images module
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1. Put CellProfiler into TestMode <imgsrc="./TutorialImages/startTestMode.png"width="120"alt="Start Test Mode button"/>, open the eye icons <imgsrc="./TutorialImages/EyeOpen.png"width="20"alt="Open Eye icon"/> next to RunCellpose and OverlayOutlines, and then hit Run <imgsrc="./TutorialImages/Run.png"width="120"alt="Run"/>
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1. Put CellProfiler into TestMode <imgsrc="./TutorialImages/startTestMode.png"width="120"alt="Start Test Mode button"/>
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1. If using Docker/Podman, and if you've already pre-downloaded a container, ensure that the `Select Cellpose Docker image` setting in the `RunCellpose` module matches the container you already downloaded.
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1. Open the eye icons <imgsrc="./TutorialImages/EyeOpen.png"width="20"alt="Open Eye icon"/> next to RunCellpose and OverlayOutlines, and then hit Run <imgsrc="./TutorialImages/Run.png"width="120"alt="Run"/>
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1. You may wish to put a pause <imgsrc="./TutorialImages/Pause.png"width="20"alt="Pause"/> next to SaveImages, or uncheck it <imgsrc="./TutorialImages/InactivatedModule.png"width="20"alt="Unchecked"/>, to keep it from saving images while you are exploring this pipeline.
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1. As before, using OverlayOutlines and/or the WorkspaceViewer, evaluate segmentation on a few images. Where is CellProfiler doing better, and where is Cellpose doing better?
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1. Use the Info <imgsrc="./TutorialImages/Info.png"width="35"alt="Info"/> button to learn more about the different parameters you can pass to Cellpose (we don't offer all of them, but many!) - how does tweaking these affect your output? How about changing the model you're using, and/or the image you're segmenting?
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