Fragpipe V23.1 crashes with DDA+ in hybrid DIA

[NikolaMonteverde]

log_2025-10-29_07-36-25.txt

Hello, sorry to bother you again but I am running Fragpipe V23.1 with Ultra2 (TiMS-TOF) data. I have 10 DDA files (8 are fractions and 2 are synthetic peptides). There are 104 DIA files. I get a crash at the DDA end where the mzBIN_calibrated folder of the fractions don't have MS1 data. Any way to fix it? Thanks

[fcyu]

You are using the database splitting feature for the DDA+ data, which is not well supported yet. Is there a way to not use the database splitting for your analysis?

Thanks,

Fengchao

[NikolaMonteverde]

Unfortunately it crashes if I don't perform database splitting. It's a rather bulky search and am searching for phospho sites so elected to use splitting. I will run in DDA for now. Any idea when database splitting will be possible with hybrid DIA with DDA+ enabled? Thanks

[fcyu]

We are working on it now. Hopefully, it will be ready soon.

Best,

Fengchao