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Contributor submission NosF #166

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77 changes: 77 additions & 0 deletions mite_data/data/c4bb829e-e56d-11f0-b8d3-8218ec668ab3.json
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{
"accession": "MITE9999999",
"status": "pending",
"comment": "Interacts with NosGH in the formation of thiazole moieties in the nosiheptide biosynthetic pathway.",
"changelog": [
{
"version": "1",
"date": "2025-12-30",
"contributors": [
"AAAAAAAAAAAAAAAAAAAAAAAA"
],
"reviewers": [
"BBBBBBBBBBBBBBBBBBBBBBBB"
],
"comment": "New entry."
}
],
"enzyme": {
"name": "NosF",
"description": "Dehydrogenase",
"references": [
"doi:10.1021/cb900133x",
"doi:10.1021/jacs.5c03922"
],
"auxiliaryEnzymes": [
{
"name": "NosG",
"description": "E1-YcaO didomain protein",
"databaseIds": {
"uniprot": "C6FX46",
"genpept": "ACR48336.1"
}
},
{
"name": "NosH",
"description": "Ocin-ThiF-like protein",
"databaseIds": {
"uniprot": "C6FX47",
"genpept": "ACR48337.1"
}
}
],
"databaseIds": {
"uniprot": "UPI0001AABFA7",
"genpept": "ACR48335.1",
"mibig": "BGC0000610"
}
},
"reactions": [
{
"tailoring": [
"Dehydrogenation"
],
"description": "Dehydrogenation of thiazolines to thiazoles in nosiheptide pathway. Current data indicates that full leader peptide is required for substrate recognition.",
"reactionSMARTS": 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"reactions": [
{
"substrate": "CCC(C)C(NC(=O)C(CC(=O)O)NC(=O)C(CC(C)C)NC(=O)C(CC(=O)O)NC(=O)C(CO)NC(=O)C(CC(C)C)NC(=O)C(Cc1c[nH]cn1)NC(=O)C(C)NC(=O)C(C)NC(=O)C(CC(=O)O)NC(=O)C(N)CCSC)C(=O)NC(CC(=O)O)C(=O)NC(C)C(=O)NC(CC(C)C)C(=O)NC(CCC(=O)O)C(=O)NC(C(=O)NC(CO)C(=O)NC(CCC(=O)O)C(=O)NC(Cc1ccccc1)C(=O)NC(CC(C)C)C(=O)NC(CC(=O)O)C(=O)NC(CCC(=O)O)C(=O)NC(CO)C(=O)NC(CCCNC(=N)N)C(=O)NC(CC(C)C)C(=O)NC(CCC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CO)C(=O)NC(CCC(=O)O)C(=O)NC(C(=O)NC(C(=O)NC(C)C(=O)NC(CCCCN)C(=O)NC(C(=O)NC(CCSC)C(=O)NC(CO)C(=O)NC(C)C(=O)NC(CO)C1=NC(C(=O)NC(C(=O)NC(C2=NC(C(=O)NC(CCC(=O)O)C3=NC(C(=O)NC(CS)C4=NC(C(=O)NC(CO)C5=NC(C(=O)NC(CO)C(=O)NC(CO)C(=O)O)CS5)CS4)CS3)CS2)C(C)O)C(C)O)CS1)C(C)C)C(C)C)C(C)C)C(C)CC",
"products": [
"CCC(C)C(NC(=O)C(CC(=O)O)NC(=O)C(CC(C)C)NC(=O)C(CC(=O)O)NC(=O)C(CO)NC(=O)C(CC(C)C)NC(=O)C(Cc1c[nH]cn1)NC(=O)C(C)NC(=O)C(C)NC(=O)C(CC(=O)O)NC(=O)C(N)CCSC)C(=O)NC(CC(=O)O)C(=O)NC(C)C(=O)NC(CC(C)C)C(=O)NC(CCC(=O)O)C(=O)NC(C(=O)NC(CO)C(=O)NC(CCC(=O)O)C(=O)NC(Cc1ccccc1)C(=O)NC(CC(C)C)C(=O)NC(CC(=O)O)C(=O)NC(CCC(=O)O)C(=O)NC(CO)C(=O)NC(CCCNC(=N)N)C(=O)NC(CC(C)C)C(=O)NC(CCC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CO)C(=O)NC(CCC(=O)O)C(=O)NC(C(=O)NC(C(=O)NC(C)C(=O)NC(CCCCN)C(=O)NC(C(=O)NC(CCSC)C(=O)NC(CO)C(=O)NC(C)C(=O)NC(CO)c1nc(C(=O)NC(C(=O)NC(c2nc(C(=O)NC(CCC(=O)O)c3nc(C(=O)NC(CS)c4nc(C(=O)NC(CO)c5nc(C(=O)NC(CO)C(=O)NC(CO)C(=O)O)cs5)cs4)cs3)cs2)C(C)O)C(C)O)cs1)C(C)C)C(C)C)C(C)C)C(C)CC"
],
"isIntermediate": true,
"description": "Thiazole-formation on modified NosM precursor peptide in nosiheptide biosynthetic pathway."
}
],
"evidence": {
"evidenceCode": [
"Heterologous expression",
"Knock-out studies"
],
"references": [
"doi:10.1021/jacs.5c03922"
]
}
}
]
}