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@a-hariti a-hariti commented Mar 13, 2025

the current syntax is deprecated as highlighted by this note from the command output:

********** NOTE: Picard's command line syntax is changing.
**********
********** For more information, please see:
********** https://github.com/broadinstitute/picard/wiki/Command-Line-Syntax-Transition-For-Users-(Pre-Transition)
**********
********** The command line looks like this in the new syntax:
**********
**********    MarkDuplicates -I SRR038564.bwa-mem.sorted.bam -O SRR038564.bwa-mem.sorted.marked.bam -M SRR038564.bwa-mem.sorted.marked.bam.metrics
**********

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